Investigation of recovery of volatiles of Bidens tripartita L. using solid-phase extraction trap in supercritical fluid extraction

Abstract

Recovery of Bidens tripartita L. volatiles using supercritical CO2 extraction with solid-phase trap was performed in this study. Three aspects were under investigation: the impact of solvent (heptane, methanol or acetonitrile) applied to rinse the analytes from the trap; the impact of the amount of plant material used for extraction; the release of volatiles from plant matrix using multiple extraction. α-Pinene, p-cymene, β-ocimene, and β-elemene were predominant in all extracts prepared in different ways. β-ocimene was the major compound (40-46%) in all extracts regardless of the solvent used. No significant difference in amount of α-pinene was observed when different trap desorption solvents were used, while heptane desorbed significantly higher amounts (12-31%) of other compounds. The volatile composition showed both qualitative and quantitative differences when different amounts of sample material were used. The extraction extent of the main compounds varied between first and repeated extractions.