The planar lipid bilayer technique is a powerful experimental approach for electrical single channel recordings of pore-forming membrane proteins in a chemically well-defined and easily modifiable environment. Here we provide a general survey of the basic materials and procedures required to set up a robust bilayer system and perform electrophysiological single channel recordings of reconstituted proteins suitable for the in-depth characterization of their functional properties. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Bartsch, P., Harsman, A., & Wagner, R. (2013). Single channel analysis of membrane proteins in artificial bilayer membranes. Methods in Molecular Biology, 1033, 345–361. https://doi.org/10.1007/978-1-62703-487-6_22
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