Laser-capture microdissection (LCM) has revolutionized the isolation of defined regions and specific cell populations from human tissue. This approach used in combination with immunohistochemistry (immuno-LCM) has become a valuable method for isolating individual cell-types from a complex heterogeneous population. Here, we describe the detailed methodology required for the isolation of enriched populations of GFAP+ astrocytes, OSP+ oligodendrocytes, and CD68+ microglia from frozen post-mortem human central nervous system tissue using immuno-LCM.
CITATION STYLE
Simpson, J. E., Wharton, S. B., & Heath, P. R. (2018). Immuno-laser-capture microdissection for the isolation of enriched glial populations from frozen post-mortem human brain. In Methods in Molecular Biology (Vol. 1723, pp. 273–284). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7558-7_16
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