A cyclic AMP response element is involved in retinoic acid-dependent RARβ2 promoter activation

Abstract

Activation of the retinoic acid receptor (RAR) β2 promoter is known to be mediated by a RA response element located in the proximity of the TATA-box. By deletion studies in P19 embryonal carcinoma cells we have analyzed the RARβ2 promoter for the presence of additional regulatory elements. We found that the cyclic AMP response element-related motif, TGATGT CA at position -99 to -92, is able to enhance RA-dependent RARβ2 promoter activation. In addition we demonstrate that this element, designated CRE-β2, is functionally active as a CRE since it can bind members of the CREB/ATF transcription factor family and, moreover, mediates the stimulatory effect of cAMP on RA-dependent RARβ2 promoter activation in human foetal kidney 293 cells. © 1992 Oxford University Press.