Cell-free circulating DNA in plasma and serum may serve as a biomarker for malignant tumor detection and follow up in patients with a variety of solid tumors including prostate cancer. In healthy patients, DNA is normally released from an apoptotic source which generates small fragments of cell-free DNA, whereas cancer patients have cell-free circulating DNA that originated from necrosis, autophagy, or mitotic catastrophe. Cell-free circulating DNA levels were measured by a quantitative real-time PCR method with a set of primers targeted to amplify the consensus ALU apoptotic versus necrotic origin. Prostate cancer patients before and 3 months after diagnosis showed cell-free circulating DNA released at apoptotic and non-apoptotic cell death. Interestingly, all patients after 6 months demonstrated DNA released at non-apoptotic cell. The principal source of cell-free circulating DNA is of apoptotic and non-apoptotic cell death. However, during treatment, this feature could change. Therefore, the study of cell-free circulating DNA would be important to follow the evolution of the disease during the treatment. © 2012 International Society of Oncology and BioMarkers (ISOBM).
CITATION STYLE
Delgado, P. O., Alves, B. C. A., De Sousa Gehrke, F., Kuniyoshi, R. K., Wroclavski, M. L., Del Giglio, A., & Fonseca, F. L. A. (2013). Characterization of cell-free circulating DNA in plasma in patients with prostate cancer. Tumor Biology, 34(2), 983–986. https://doi.org/10.1007/s13277-012-0634-6
Mendeley helps you to discover research relevant for your work.