It has been demonstrated that microfluidic systems allow integration of sampling, reagent mixing, and rapid electrophoretic analysis. They have also proven useful for culturing cells wherein control over the environment allows novel and automated experiments. Here, we describe a microchip-based electrophoresis assay that allows cell culture and hormone monitoring. An online gradient generator can control cell culture condition precisely. This system has been applied for Pancreas islets’ glucose sensitivity studies.
CITATION STYLE
Lu, S., & Kennedy, R. T. (2017). Using electrophoretic immunoassay to monitor hormone secretion. In Methods in Molecular Biology (Vol. 1547, pp. 57–67). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6734-6_5
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