Effect of ozone and doxorubicin on the viability and morphology of malignant hepatic cells

Abstract

The aim of the investigation was to study the effect of isolated and combined action of ozone-oxygen mixture and doxorubicin on the viability and morphology of normal and malignant human hepatic cells. Material and Methods. The culture of normal (Chang liver) and malignant (SK-HEP-1) human liver cells were used in the study. Doxorubicin and ozone-oxygen gas mixture were introduced to the culture medium for cell growth. Viability was tested by reduction of MTT tetrazole salts. Morphological investigations were carried out 48 h after the replacement of the cultural medium using DMIL HC inverted microscope (Germany). Results. Doxorubicin possesses the highest cytotoxicity relative to both normal and malignant human hepatic cells. Ozone alone and in combination with doxorubicin also exerts a marked cytotoxic effect on the viability of the cells and results in irreversible consequences for the structure of the cell elements. The results obtained can be used for selection of ozone doses and ways of ozone introduction in various tumor localizations.