GC-MS analysis of phytocomponents in the ethanolic extract of Nelumbo nucifera seeds from Russia

Abstract

The present study was designed to determine the phytocomponents of the ethanol extract of N. nucifera seed using GC-MS. The phytochemical screening of the ethanolic extract of N. nucifera seed was performed using Perkin-Elmer Gas Chromatography - Mass Spectroscopy. GC-MS chromatogram of the ethanolic extract of N. nucifera seed showed thirty-eight peaks which indicating the presence of thirty-eight phytochemical constituents. The major phytochemical constituents are lupeol (22.95%), octadecatrienoic acid-2-[(trimethylsilyl)oxy]-1- [[(trimethylsilyl)oxy] methyl]ethyl ester (Z,Z,Z) (7.86%), 2,3-dihydroxypropyl--cis-13-docosenoate (5.35%), oleanolic acid(5.19%), tristrimethylsilyl ether derivative of 1,25-dihydroxyvitamin D2 (3.36%) ,trimethylsilyl derivative of 2-monoolein (3.26%), lucenin 2 (3.02%), lupanol (2.95%), tetraneurin-a-diol (2.78%), carotene- 1,1',2,2'-tetrahydro-1,1'-dimethoxy (2.32%), 1-oxo-forskolin (1.99%), oleic acid-3-hydroxypropyl ester (1.78%), oleic acid trimethylsilyl ester (1.69%), betulin (1.61%), à-amyrin-trimethylsilyl ether (1.49%), stigmast-5-en-3- ol-(3á,24S)(1.43%), flavone-4'-OH,5-OH,7-di-O-glucoside (1.11%). Among thirty-eight compounds identified, only twelve were reported to have biological activities. Our results indicate that N. nucifera seed contain various bioactive components.