Estimation of the role of apoptosis in altering the architecture of the cells of exocrine pancreas in mice inoculated with mammary gland carcinoma by using soft laser

Abstract

Twenty four adult female of Swiss white mice (aged 60 days each) and weighing 42-48gm were selected for this experimental study. They were divided into four groups (4 mice each) A, B, C, and D inoculated with mammary gland carcinoma and irradiated with laser, they were considered as irradiated groups of mice. The remaining animals were divided also into four groups a, b, c, and d inoculated with mammary gland carcinoma not irradiated with laser, they were considered as control groups of mice. The laser of 650nm, 50 mw power, 25 mw/cm2 power density was focused on the pancreas of the targeted mice which represent irradiated groups. The laser caused a reduction in the damage that occurred in the architecture of the cells of exocrine pancreas because of the negative effects caused by cancer on the mentioned cells such as decreased in size, dense fragmentation of the content of the nucleus, and dark appearance of the cytoplasm which represent slight degenerative alterations. In the same time, the laser caused a decrease in the mean of the apoptotic cells of the exocrine pancreas gradually for the same reason that above mentioned. Whereas in the control groups of mice, the mean of apoptotic cells of the exocrine pancreas displayed a gradual increase because the cancer caused activation in cellular apoptosis. Besides, there were diagnostic markers in the examination of the histology of the cells of exocrine pancreas which showed that there were abundant degenerative alterations in the architecture of the cells such as decreased in size, dense fragmentation of the content of the nucleus, and dark appearance of the cytoplasm because of the suppressive activity of the cancer which caused these damages in the architecture of the cells.