Assessment of salivary free cortisol levels by liquid chromatography with tandemass spectrometry (LC-MS/MS) in patients treated with mitotane

16Citations
Citations of this article
29Readers
Mendeley users who have this article in their library.

Abstract

Objective: Mitotane is an adrenocytolytic agent used in adrenocortical carcinoma, inducing adrenal insufficiency, requiring replacement treatment. Such therapy is not easy to monitor because of mitotane interference. Salivary cortisol reflects a free fraction of plasma cortisol and may be useful in such patients. Design: The aim of our study was to evaluate salivary cortisol by HPLC coupled to tandem-mass spectrometry (LC-MS/MS) and by an electrochemiluminescence immunoassay (ECLIA) in patients treated with mitotane. We enrolled 6 patients receiving mitotane and 2 Addison disease patients as negative controls and determined salivary cortisol rhythm. We also determined the salivary cortisol rhythm in 8 healthy subjects. Salivary samples (n=112) were assayed by ECLIA, using Roche Modular E170, and by LC-MS/MS. Results: The mean values obtained by ECLIA were significantly higher than those obtained by LC-MS/MS in the mitotane group (p<0.001). In fact, in the group measured by LC-MS/ MS, we observed several peaks eluting at a retention time different from the cortisol group, presumably due to cortisol-like analogues. In Addison disease, since steroidogenesis is absent, salivary cortisol values measured by the two methods did not show any significant difference (p=0.61). Conclusions: Salivary cortisol measured by LC-MS/MS is a selective method, excluding cortisol analogues accumulating in treated patients. Therefore, LC-MS/MS offers an effective system to monitor replacement therapy in mitotane treated patients.

Cite

CITATION STYLE

APA

Carrozza, C., Lapolla, R., Gervasoni, J., Rota, C. A., Locantore, P., Pontecorvi, A., … Persichilli, S. (2012). Assessment of salivary free cortisol levels by liquid chromatography with tandemass spectrometry (LC-MS/MS) in patients treated with mitotane. Hormones, 11(3), 344–349. https://doi.org/10.14310/horm.2002.1363

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free