Berberine induces apoptosis and DNA damage in MG-63 human osteosarcoma cells

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Abstract

Berberine, an isoquinoline alkaloid extracted from the dry root of Coptidis Rhizoma, has been found to exhibit marked anticancer effects on a panel of established cancer cells. Among the human osteosarcoma lines treated, MG-63 cells were found to be the most sensitive. The present study investigated the potential genotoxic effect of berberine on MG-63 human osteosarcoma cells. The effect of berberine on cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell apoptosis was analyzed by flow cytometry and a DNA ladder assay. γH2AX focus formation was used to detect DNA damage in MG-63 cells. Berberine induced a significant increase in apoptosis in MG-63 cells in a concentration- and time-dependent manner, as determined by DNA fragmentation analysis and flow cytometry. Furthermore, berberine induced significant concentration- and time-dependent increases in DNA damage compared with that in the negative control. In conclusion, these observations indicated that berberine induced apoptosis and DNA damage in MG-63 cells.

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Zhu, Y., Ma, N., Li, H. X., Tian, L., Ba, Y. F., & Hao, B. (2014). Berberine induces apoptosis and DNA damage in MG-63 human osteosarcoma cells. Molecular Medicine Reports, 10(4), 1734–1738. https://doi.org/10.3892/mmr.2014.2405

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