Analysis of Wnt pathway genes during ex vivo expansion and neutrophil differentiation of umbilical cord-blood derived CD34+ cells

Abstract

Previous work has shown that optimal ex vivo expansion and differentiation of CD34+ progenitor cells into neutrophils is by addition of Flt3-L, SCF and G-CSF. Here we report that a variety of genes involved in the WNT pathway are transcriptionally active in both undifferentiated and differentiated umbilical cord blood CD34+ cells, however statistically significant changes in gene expression are not always consistent across UCB samples. © 2010 International Society of Blood Transfusion.