P450 oxidoreductase: Genotyping, expression, purification of recombinant protein, and activity assessments of wild-type and mutant protein

Abstract

P450 oxidoreductase (POR) is the flavoprotein that transfers electrons from NADPH to microsomal cytochrome P450 enzymes and to some other proteins. Protocols for genotyping human POR for common polymorphisms are described. Expression in E. coli of recombinant human POR, its puri fication, and different methods of assessing the effect of amino-acid sequence variants of POR on the activity of various cytochromes P450 are also described. © Springer Science+Business Media New York 2013.