Super-resolution Radial Fluctuations (SRRF) imaging is a computational approach to fixed and live-cell super-resolution microscopy that is highly accessible to life science researchers since it uses common microscopes and open-source software plugins for ImageJ. This allows users to generate super-resolution images using the same equipment, fluorophores, fluorescent proteins and methods they routinely employ for their studies without specialized sample preparations or reagents. Here, we discuss a step-by-step workflow for acquiring and analyzing images using the NanoJ-SRRF software developed by the Ricardo Henriques group, with a focus on imaging chromatin. Increased accessibility of affordable super-resolution imaging techniques is an important step in extending the reach of this revolution in cellular imaging to a greater number of laboratories.
CITATION STYLE
Salsman, J., & Dellaire, G. (2022). Super-Resolution Radial Fluctuations (SRRF) Microscopy. In Methods in Molecular Biology (Vol. 2440, pp. 225–251). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2051-9_14
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