Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.
CITATION STYLE
Knipscheer, P., Räschle, M., Schärer, O. D., & Walter, J. C. (2012). Replication-Coupled DNA Interstrand Cross-Link Repair in Xenopus Egg Extracts (pp. 221–243). https://doi.org/10.1007/978-1-61779-998-3_16
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