BEE VENOM DRUG POTENTIALITY ON THE MACROMOLECULES DAMAGE OF THE LARVAL GUT OF HERMETIA ILLUCENS (L.), (DIPTERA: STRATIOMYIDAE)

Abstract

Bee venom is a complex mixture of apamin, mast-cell degranulating peptide, phospholipase A2 and melittin proteins, which are responsible for multiple actions in biochemical reaction of different living organisms including insect. Black soldier fly (Hermetia illucens (L.), larvae have gained popularity both for their ability to decompose organic waste and serve as a source of proteins for domestic. Use of black soldier fly larvae (BSFL) is among the solutions being explored to shift the value chain in organic waste management by producing valuable products. Monitoring of possible impact of bee venom was assessed using mortality rate and biochemical parameters such as protein carbonyls amount, and lipid peroxides concentration. The potential use of protein carbonylation, and lipid peroxidation as a biomonitoring method of bee venom potentiality was proposed. Concentrations of protein carbonyls of H. illucens gut samples treated with 1mg/ml bee venom are significantly increased than control samples. The lipid peroxidation level in gut of 5 th instar insect treated with 1, 0.5, 0.25, & 0.125mg/ml were 65, 57, 46, & 34, respectively, compared to control. Bee venom specific antioxidant activity caused comparable adverse effects in the organisms inhabiting concentration up to 1mg/ml.