Fluorescence cross-correlation spectroscopy of plant proteins

Abstract

Fluorescence cross-correlation spectroscopy (FCCS) is a technique that physically evaluates the molecular interaction between two fluorophore-tagged molecules such as proteins and oligonucleotides in a quantitative manner. Because it simply makes use of the coincidental movement of two molecules, it could avoid the complexity that sometimes occurs in other fluorescent techniques such as fluorescence resonance energy transfer. The present chapter describes procedures for FCCS of plant proteins expressed and measured in HeLa cells. Determination in plant cells is also mentioned briefly. © 2009 Humana Press.