Proteomics is one of the key approaches to understand plant cell physiology involving the regulation of expression of many genes and metabolite production. Technical advances allowed a deeper characterization of plant proteomes, highlighting the need to study cellular compartments. The apoplast is the cellular compartment external to the plasma membrane including the cell wall, where a broad range of processes take place including intercellular signaling, metabolite transport, and plant–microbe interactions. Due to the fragile nature of leaf tissues, it is a challenge to obtain apoplastic fluids from leaves while maintaining cell integrity, which is particularly true for woody plants. Here, we describe the vacuum infiltration-centrifugation (VIC) method for the extraction of the apoplastic fluid compatible with high-throughput proteomic approaches and biochemical analysis from different woody plants.
CITATION STYLE
Figueiredo, A., & Guerra-Guimarães, L. (2021). Isolation of Apoplastic Fluid from Woody Plant Leaves: Grapevine and Coffee as a Case Study. In Methods in Molecular Biology (Vol. 2259, pp. 49–57). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1178-4_4
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