Single-cell mRNA sequencing can dissect heterogeneous cell populations as it can identify cell types and cellular states based on their unique transcriptional signatures. We use fluorescence-activated cell sorting (FACS) to isolate individual cultured neurons derived from human-induced pluripotent stem cells (hiPSCs) followed by polyA-based Smart-Seq2 RNA sequencing to analyze the single-cell transcriptional profiles. We provide protocols and guidelines on dissociation, cell selection, and library preparation that can be readily adapted to other cell types or tissue samples.
CITATION STYLE
Schweingruber, C., Nijssen, J., Benitez, J. A., & Hedlund, E. (2023). Single-Cell mRNA-Seq of In Vitro-Derived Human Neurons Using Smart-Seq2. In Methods in Molecular Biology (Vol. 2594, pp. 143–164). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2815-7_11
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