The importance of glycans in various cellular events, especially intracellular and intercellular trafficking of proteins, has been reported in numerous studies. Here, we present a novel method to monitor endocytosis of proteins of interest bearing a specific glycan modification. Using a fl uorescence resonance energy transfer technique, we investigated the role of glycan structure on the internalization of insulin-responsive glucose transporter GLUT4. We found that sialylated glycoforms of GFP-tagged GLUT4 appear to be internalized more slowly than non-sialylated GLUT4 upon insulin removal. This novel glycan imaging tool allows probing functional roles of specific glycan modifications in endocytosis of various proteins.
CITATION STYLE
Haga, Y., & Suzuki, T. (2014). Use of transmembrane FRET to investigate the internalization of glycosylated proteins. Methods in Molecular Biology, 1174, 225–230. https://doi.org/10.1007/978-1-4939-0944-5_15
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