Innovations in the Insect Cell Expression System for Industrial Recombinant Vaccine Antigen Production

26Citations
Citations of this article
102Readers
Mendeley users who have this article in their library.

Abstract

The insect cell expression system has previously been proposed as the preferred biosecurity strategy for production of any vaccine, particularly for future influenza pandemic vaccines. The development and regulatory risk for new vaccine candidates is shortened as the platform is already in use for the manufacturing of the FDA-licensed seasonal recombinant influenza vaccine Flublok®. Large-scale production capacity is in place and could be used to produce other antigens as well. However, as demonstrated by the 2019 SARS-CoV-2 pandemic the insect cell expression system has limitations that need to be addressed to ensure that recombinant antigens will indeed play a role in combating future pandemics. The greatest challenge may be the ability to produce an adequate quantity of purified antigen in an accelerated manner. This review summarizes recent innovations in technology areas important for enhancing recombinant-protein production levels and shortening development timelines. Opportunities for increasing product concentrations through vector development, cell line engineering, or bioprocessing and for shortening timelines through standardization of manufacturing processes will be presented.

References Powered by Scopus

Production of human beta interferon in insect cells infected with a baculovirus expression vector.

760Citations
N/AReaders
Get full text

On the classification and nomenclature of baculoviruses: A proposal for revision

484Citations
N/AReaders
Get full text

Antibody production

442Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Genetic engineering of baculovirus-insect cell system to improve protein production

44Citations
N/AReaders
Get full text

CCHFV vaccine development, current challenges, limitations, and future directions

14Citations
N/AReaders
Get full text

Comparison of chemometric models using Raman spectroscopy for offline biochemical monitoring throughout the VLP-making upstream process

11Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Cox, M. M. J. (2021, December 1). Innovations in the Insect Cell Expression System for Industrial Recombinant Vaccine Antigen Production. Vaccines. MDPI. https://doi.org/10.3390/VACCINES9121504

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 28

70%

Researcher 8

20%

Lecturer / Post doc 3

8%

Professor / Associate Prof. 1

3%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 21

55%

Agricultural and Biological Sciences 8

21%

Immunology and Microbiology 5

13%

Chemical Engineering 4

11%

Save time finding and organizing research with Mendeley

Sign up for free