Abstract
A gene library of Clostridium acetobutylicum ATCC824 was constructed in the plasmid vector pEcoR251. The library was tested for the presence of starch hydrolyzin clones. One clone in which the recombinant plasmid, pVP101, conferred α-amylase activity to the Escherichia coli host cell, was detected. The gene is carried on a 3.45-kbp BglII restriction fragment. A detailed physical map of pVP101 is presented. © 1989.
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Verhasselt, P., Poncelet, F., Vits, K., Van Gool, A., & Vanderleyden, J. (1989). Cloning and expression of a Clostridium acetobutylicum α-amylase gene in Escherichia coli. FEMS Microbiology Letters, 59(1–2), 135–140. https://doi.org/10.1111/j.1574-6968.1989.tb03097.x
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