The genetic variation and stability analysis of rice mutant lines using AMMI model under normal and drought stress conditions

Abstract

This study was conducted to investigate the genotype × environment interaction and stability analysis of 14 mutant lines (with their parental lines). The study was designed for two years, 2015-2016, in two environmental conditions, normal and drought stress, in Rasht. Based on the results of combined analysis of variance, genotype, environment, and genotype × environment effects were significant at 1% probability level. The significance of the environmental effect indicates that the environments were different in terms of genotypes performance. Therefore, the stability analysis was performed with different methods of measuring stability. The results of ANOVA for the additive main effect and multiplicative interaction (AMMI) showed the genotype and environment main effects and genotype × environment effects were significant at 1% probability level. Based on the AMMI results, TM6-230-VE-7-5-1, TM6-230-VE-8-4-1, Khazar, and KM5-200-4-2-1 had a positive and negative interaction, respectively. HM5-300-5-1, HM5-250-12-1, TM6-B-19-2 were selected as the stable genotypes. Stability analysis determined through AMMI stability value (ASV) method showed HM5-250-12-1, Tarom, HM5-250-E-1-1, TM6-B-19-2, Hashemi and HM5-300-5-1 with the least stability value were introduced as the most stable cultivars.