PrimerProspector: De novo design and taxonomic analysis of barcoded polymerase chain reaction primers

Abstract

Motivation: PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task. Results: PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data. © The Author(s) 2011. Published by Oxford University Press.