MicroRNA-329-3p targets MAPK1 to suppress cell proliferation, migration and invasion in cervical cancer

Abstract

Cervical cancer is the second most common gynecological cancer worldwide and remains as one of the leading causes of cancer-related death among women. Despite great progress in the treatment of cervical cancer, the 5-year overall survival rate for patients with this disease remains unsatisfactory. Over the past decade, an increasing number of studies indicate a central role for microRNAs in the initiation and progression of cervical cancer. microRNA-329-3p (MIR-329-3p) has been studied in many types of human cancer; however, the expression level, biological role and the underlying mechanism of MIR-329-3p in cervical cancer has not yet been investigated. In the present study, we found that the expression levels of MIR-329-3p were reduced in both cervical cancer tissues and cell lines. Low MIR-329-3p expression was negatively correlated with histological grade, International Federation of Gynecology and Obstetrics (FIGO) stage, and lymph node metastasis of cervical cancer patients. In addition, upregulation of MIR-329-3p suppressed cell proliferation, migration and invasion of cervical cancer. Furthermore, MAPK1 was identified as a direct target gene of MIR-329-3p. MAPK1 was significantly upregulated in cervical cancer tissues and was inversely correlated with MIR-329-3p expression in the cervical cancer tissues. Silencing of MAPK1 by RNA interference mimicked the effects of MIR-329-3p overexpression on cell proliferation, migration and invasion in cervical cancer. Moreover, rescue experiments showed that restoration of the expression of MAPK1 reversed the effects of MIR-329-3p overexpression in cervical cancer cells. Taken together, these findings suggest that MIR-329-3p has a critical tumor-suppressive roles by directly targeting MAPK1 in cervical cancer, and it may be investigated as a novel therapeutic target for the treatment of patients with this disease.