Human platelet antigen (HPA) typing plays a critical role in the diagnosis of fetal/neonatal alloimmune thrombocytopenia, and the prevention of posttransfusion purpura and refractoriness to platelet transfusions. The recent development of high-throughput genotyping methods, allowing simultaneous genotyping of as many as 17 HPAs, is of utmost interest for saving time and money. Here, we describe a microarray technology named “BeadChip,” designed for HPA-1 to -9, -11, and -15 genotyping of up to 96 individuals, in approximately 5 h. This technology was used to study allele frequencies in Brazilian blood donors, considering the heterogeneous ethnic composition.
CITATION STYLE
Bertrand, G., & Conti, F. (2015). Genotyping of human platelet antigens by BeadChip microarray technology. Methods in Molecular Biology, 1310, 149–165. https://doi.org/10.1007/978-1-4939-2690-9_13
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