BRCA1 promoter methylation status in ovarian cancer

Abstract

Objective: To evaluate the methylation status of the breast cancer 1, early onset (BRCA1) promoter in ovarian cancer in Iranian patients. Methods: The BRCA1 promoter methylation status of tissue from 60 patients with Müllerian-type ovarian cancer and matched benign ovarian tissue from the same patients was evaluated using bisulfate-modified DNA in methylation-specific polymerase chain reaction (MSP CR) assays. Results: Analysis of BRCA1 promoter methylation status showed that 8 of 60 cases (13%) were methylated and 11 of 60 cases (18%) were unmethylated. Conclusion: Methylation of the BRCA1 promoter may be a risk factor or cause of ovarian cancer. © 2012 by The American Society for Clinical Pathology.