Flow cytometric analysis of stat phosphorylation

Abstract

Flow cytometry can be used to study STAT phosphorylation on a per cell basis. Cells are fixed, permeablized, and stained with antibodies that specifically bind to the phosphorylated form of the STAT protein. This allows the tyrosine phosphorylation of a single STAT to be studied within a heterogeneous cell population and/or the phosphorylation of several STATs within the one cell type.