Immunoblotting normalization issues have been recently overcome by whole lane staining. Herein, we are taking advantage of these recent advances and of the fluorophore status of the Ponceau S stain in order to combine the advantages of whole lane staining and fluorescence. By Ponceau S excitation at 488 nm, we identify the so-called ‘fluorescent Ponceau’ method as more linear, more sensitive and more repeatable than the others in protein lysates of distant biochemical profiles (cells, human and mouse tissues). This essentially cost-free method at the single experiment level provides accessible and robust means for post-blot normalization of many types of analytes.
CITATION STYLE
Verzeroli, C., Hernandez, C. A., Zoulim, F., & Parent, R. (2023). A fluorescent Ponceau S-based total protein normalization method for conventional and challenging immunoblot samples. Analytical Biochemistry, 681. https://doi.org/10.1016/j.ab.2023.115330
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