Identification of eosinophils in whole blood samples by flow cytometry is often problematic. There are usually only a low number and percentage of cells that may be detected, and it may be difficult to discriminate eosinophils from other granulocytes. Here, we propose a simple approach using the eosinophil's intrinsic autofluorescence properties, combined with detection of CCR3 expression, to reliably identify eosinophils in a mixed leukocyte population. © Springer Science+Business Media New York 2014.
CITATION STYLE
Ethier, C., Lacy, P., & Davoine, F. (2014). Identification of human eosinophils in whole blood by flow cytometry. Methods in Molecular Biology, 1178, 81–92. https://doi.org/10.1007/978-1-4939-1016-8_8
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