Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil

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Abstract

Introduction: This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil. Methods: Two hundred and ten females of 13 sand fl y species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing. Results: PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specifi c PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively. Conclusions: Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil.

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Ferreira, T. de S., Minuzzi-Souza, T. T. C., de Andrade, A. J., Coelho, T. O., Rocha, D. de A., Obara, M. T., … Gurgel-Gonçalves, R. (2015). Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil. Revista Da Sociedade Brasileira de Medicina Tropical, 48(6), 776–779. https://doi.org/10.1590/0037-8682-0076-2015

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