Methods for Assessing Fertility in C. elegans from a Single Population

Abstract

Reproductive senescence occurs in a wide range of species with mechanistic aspects that are conserved from Caenorhabditis elegans to humans. Genetic and environmental factors can influence fertility and reproductive output can impact rates of aging. The C. elegans Bristol N2 strain commonly used in laboratories is hermaphroditic, producing a defined number of sperm during larval development before switching exclusively to oogenesis. Here we show a method of assaying both oocyte and sperm quality from a single population of animals.