Green synthesis of zinc oxide nanoparticles using Hibiscus subdariffa leaf extract: Effect of temperature on synthesis, anti-bacterial activity and anti-diabetic activity

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Abstract

Zinc oxide (ZnO) nanoparticles (NPs) have been synthesized using Hibiscus subdariffa leaf extract. Temperature dependent synthesis and particle growth have been studied. Formation of NPs was confirmed by UV-visible (UV-VIS) spectroscopy, Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD). Electron microscopy has been used to study the morphology and size distribution of the synthesized particles. The synthesized ZnO nanoparticles as potential anti-bacterial agents have been studied on Escherichia coli and Staphylococcus aureus. Another study has indicated that small sized ZnO NPs, stabilized by plant metabolites had better anti-diabetic effect on streptozotocin (STZ) induced diabetic mice than that of large sized ZnO particles. It has also been observed by enzyme linked immunosorbent assay (ELISA) and real time polymerase chain reaction (RT-PCR) that ZnO can induce the function of Th1, Th2 cells and expressions of insulin receptors and other genes of the pancreas associated with diabetes.

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Bala, N., Saha, S., Chakraborty, M., Maiti, M., Das, S., Basu, R., & Nandy, P. (2015). Green synthesis of zinc oxide nanoparticles using Hibiscus subdariffa leaf extract: Effect of temperature on synthesis, anti-bacterial activity and anti-diabetic activity. RSC Advances, 5(7), 4993–5003. https://doi.org/10.1039/c4ra12784f

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