Ultraviolet-B radiation alters phenolic salicylate and flavonoid composition of Populus trichocarpa leaves

Abstract

We investigated foliar phenolic composition of field- and greenhouse-grown Populus trichocarpa Torr. & A. Gray (black cottonwood) ramets subjected to near zero (0x), ambient (1x) or twice ambient (2x) concentrations of biologically effective ultraviolet-B (UV-B) radiation. After a 3-month treatment period, several age classes of foliage samples were harvested and the phenolic compounds extracted, separated by high performance liquid chromatography and identified and quantified by diode-array spectrometry and mass spectrometry. Foliar phenolic concentration was greater in 1x- and 2x-treated tissue than in 0x-treated tissue. Phenolic compounds that increased in response to UV-B radiation were predominantly flavonoids, primarily quercetin and kaempferol glycosides. Enhancement of UV-B radiation from 1 x to 2x ambient concentration did not result in further flavonoid accumulation in either greenhouse or field ramets; however, a non-flavonoid phenolic glycoside, salicortin, increased in response to an increase in UV-B radiation from 1x to 2x ambient concentration. Increased salicortin concentrations accounted for at least 30-40% of the total (5%) increase in UV-absorption potential of 2x-treated tissue. Because salicortin and other salicylates are important in plant-herbivore-predator relationships, these increases are discussed in the context of collateral feeding studies. We conclude that enhanced solar UV-B radiation may significantly alter trophic structure in some ecosystems by stimulating specific phenolic compounds.