RNA pulldown protocol for in vitro detection and identification of RNA-associated proteins

Abstract

Recent advances in genomics have revealed that cells encode thousands of noncoding RNA molecules that do not require translation to proteins to perform their biological roles. Although very little is known about the mechanisms of function of noncoding RNAs, these undoubtedly involve the interaction with multiple cellular proteins. Here we describe a detailed RNA pulldown protocol for the in vitro detection of proteins specifically interacting with a long RNA molecule.