In vivo measurement of H2S, polysulfides, and “SSNO− Mix”-Mediated vasoactive responses and evaluation of ten hemodynamic parameters from rat arterial pulse waveform

Abstract

The chapter describes protocols and pitfalls in in vivo studies of drug effects on anesthetized rats. It focuses on the preparation of Na2S, Na2S4, and “SSNO− mix” solutions for rat intravenous administration, surgical preparation of jugular vein for drug administration, and preparation of carotid and tail arteries for recording of arterial pulse waveform (APW) at high resolution. It describes evaluation of ten hemodynamic parameters from APW and measurement of apparent pulse wave velocity.