DNA strand breakage activity and mutagenicity of the product (s) formed by a reaction of thiamine and nitrite under simulated gastric conditions were examined. A reaction mixture of thiamine and nitrite at pH 3 induced DNA single-strand breaks when incubated with a supercoiled Plasmid DNA at pH 7.4 and 37°C. Product (s) from thiamine and nitrite were found mutagenic to Salmonella typhimurium TA100 strain without metabolic activation. The number of His+ revertant colonies was 1200/10-µmol eq of thiamine. Hence, thiamine is an undesirable precursor of DNA strand breakage and mutagenic substance (s) when mixed with nitrite. © 1995, The Pharmaceutical Society of Japan. All rights reserved.
CITATION STYLE
Hiramoto, K., Ni-iyama, H., Kato, T., & Kikugawa, K. (1995). Thiamine Is a Precursor of DNA Strand Breakage and Mutagenic Substance (s) When Mixed with Nitrite. Japanese Journal of Toxicology and Environmental Health, 41(6), 447–451. https://doi.org/10.1248/jhs1956.41.447
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