Micropropagation and genetic fidelity of the regenerants of Aglaonema ‘valentine’ using randomly amplified polymorphic DNA

Abstract

The present study reports a simple protocol for in vitro regeneration of Aglaonema ‘Valentine’ using axillary shoot explants for rapid multiplication and production of true-to-type plants. Different concentrations of benzyladenine (BA; 0, 1, 3, 5, and 7 mg·L-1), kinetin (Kin; 0, 1, 3, 5, and 7 mg·L-1), thidiazuron (TDZ; 0, 0.5, 1.0, 1.5, and 2.0 mg·L-1), naphthalene acetic acid (NAA; 0, 0.5, and 1.0 mg·L-1), and indole-3-butyric acid (IBA; 0, 0.5, and 1.0 mg·L-1) were used for shoot regeneration. The highest shoot proliferation (5.0) was obtained on Murashige and Skoog (MS) medium supplemented with 1.5 mg·L-1 TDZ and 1 mg·L-1 NAA. In vitro rooting was easily achieved with 100% at all concentrations of NAA and IBA supplemented to half- or fullstrength MS medium. Regenerated plantlets were acclimatized in greenhouse with 100% survival rate. Randomly amplified polymorphic DNA (RAPD) analysis confirmed the genetic fidelity of the regenerated plantlets and mother plant.