The f-actin-binding mprip forms phase-separated condensates and associates with pi(4,5)p2 and active rna polymerase ii in the cell nucleus

Abstract

Here, we provide evidence for the presence of Myosin phosphatase rho‐interacting protein (MPRIP), an F‐actin‐binding protein, in the cell nucleus. The MPRIP protein binds to Phospha-tidylinositol 4,5‐bisphosphate (PIP2) and localizes to the nuclear speckles and nuclear lipid islets which are known to be involved in transcription. We identified MPRIP as a component of RNA Polymerase II/Nuclear Myosin 1 complex and showed that MPRIP forms phase‐separated conden-sates which are able to bind nuclear F‐actin fibers. Notably, the fibrous MPRIP preserves its liquid-like properties and reforms the spherical shaped condensates when F‐actin is disassembled. More-over, we show that the phase separation of MPRIP is driven by its long intrinsically disordered region at the C‐terminus. We propose that the PIP2/MPRIP association might contribute to the regulation of RNAPII transcription via phase separation and nuclear actin polymerization.