Organismal energy homeostasis is maintained by complex interorgan communications making the discovery of novel drugs against metabolic diseases challenging using traditional high-throughput approaches in vitro. Here, we describe a method that rapidly identifies small molecules with an impact on organismal energy balance in vivo. Specifically, we developed a whole-organism screen for modulators of fasting metabolism using transgenic bioluminescence-reporter zebrafish for the gluconeogenic gene phosphoenolpyruvate- carboxykinase 1 (pck1).
CITATION STYLE
Gut, P., & Stainier, D. Y. R. (2015). Whole-organism screening for modulators of fasting metabolism using transgenic zebrafish. Methods in Molecular Biology, 1263, 157–165. https://doi.org/10.1007/978-1-4939-2269-7_12
Mendeley helps you to discover research relevant for your work.