Development and evaluation of some metronidazoleloaded collagen supports designed for periodontitis

Abstract

Periodontitis is one of the most common chronic disease in the world affecting both the adults and young people, having severe consequences at cardiovascular level, and causing the premature birth. Moreover, the untreated periodontal disease is the major cause for tooth loos. Thus, the purpose of this study was to design and investigate some collagen supports with metronidazole and strontium ranelate as a treatment option for bone dental regeneration and an effective way to remove the pathogen agents incriminated in the periodontal disease. Type I fibrillar collagen gel was extracted from calf hide. Collagen hydrogels with various ratios of metronidazole and strontium ranelate were rheologically tested at two temperatures: 23°C and 37°C. The collagenic supports obtained by hydrogels lyophilization were investigated by goniometric analysis. The in vitro metronidazole release from spongious matrices was conducted with a sandwich device adapted to a dissolution equipment. The hydrogels presented a pseudoplastic behaviour facilitating the formulation flow and their good manipulation. The Power law model fitted well the kinetic data indicating a non-Fickian drug transport mechanism. The physico-chemical properties were in relation with the drug release patterns from spongious supports. Based on the results obtained, we could conclude that the designed formulations are potentially usable as a favorable solution in periodontal disease.