Isolation of DNA from plants high in polyphenolics

Abstract

DNA suitable for digestion with restriction endonucleases has been isolated from Theobroma cacao, a plant high in polyphenolic compounds, and several other plant taxa using the method presented in this paper. The method relies on concentrating nuclei away from most of the cytoplasmic components and inhibiting the formation of oxidized polyphenolic compounds and their interaction with the DNA fraction in the remaining steps. © 1990 Kluwer Academic Publishers.