Biological degradation of aflatoxin B1 by Emericellopsis sp. 1912 and Sarocladium sp. 10A

Abstract

Aflatoxin B1 is highly toxic secondary metabolites predominantly produced by some fungi and commonly found in cereals worldwide, which seriously threatens the health of human and animals due to its highly toxic. The aim of this study was to isolate AFB1 degradation microorganisms from decomposed soil using coumarin medium as sole carbon source. The soil sample was inoculated in coumarin medium at 30 °C. After 7 days, the strains which grow in coumarin medium were transferred into the fresh coumarin medium for the further screening. The screening result showed that the strain 1912 and 10A could grow on the coumarin medium, and their AFB1 degradation abilities were further analyzed by high-performance liquid chromatography. Strain 1912 and 10A could degrade AFB1 rapidly and effectively, the AFB1 degradating ratio were 60.49 and 45.51% after 24 h, respectively. Based on BLAST analysis, strain 1912 belongs to the Emericellopsi sp. and strain 10A belongs to the Sarocladium sp. Respectively. This study provided a potential solution for dealing with AFB1 contamination in human diet and animal feed.