Persistent virus-induced gene silencing in asymptomatic accessions of arabidopsis

Abstract

Coupled with the advantages afforded by the model plant Arabidopsis , virus-induced gene silencing (VIGS) offers a rapid means to assess gene function. The geminivirus vector based on Cabbage leaf curl virus described here has the benefi ts of small insert size and persistent silencing of the target gene through the life cycle of the plant. Here, we show that genetic variation in the vast collection of Arabidopsis accessions can be leveraged to ameliorate viral symptomology that accompanies the VIGS procedure. The plasticity of phenotypes under different day lengths or temperature conditions can be exploited to achieve maximum silencing effi cacy in either vegetative or infl orescence tissue, according to the question being asked. Protocols and vectors for Agro -infi ltration of primary leaves, subapical pricking in older plants, and microprojectile bombardment are described.