We described a solid-supported desulfurization procedure allowing easy access to the sequence analysis of oligonucleotide phosphorothioates. The described method is based upon selective removal of the 2-cyanoethyl phosphate protecting groups, followed by iodine-promoted desulfurization of the resulting phosphorothioate diesters. Automatic oxidation of oligonucleotide phosphorothioates, anchored via an ester linkage to a standard solid support (LCAA/CPG), is combined with Maxam-Gilbert solid-support sequencing. The overall procedure allows rapid simultaneous sequence analysis of several oligonucleotide analogs.
CITATION STYLE
Jacobson, L., & Kanber, B. (2015). Genetic Algorithms in Java Basics. Genetic Algorithms in Java Basics. Apress. https://doi.org/10.1007/978-1-4842-0328-6
Mendeley helps you to discover research relevant for your work.