Mechanism of anucleate cell production in the oriC-deleted mutants of bacillus subtilis

21Citations
Citations of this article
16Readers
Mendeley users who have this article in their library.

Abstract

We constructed oriC-deleted mutants of Bacillus subtilis by integrating the minimal replication region of plasmid pLS32 into the proA (115°), spoIIIJ (360°) and thrS (256°) loci of the chromosome, respectively. All three mutants produced anucleate cells and the DNA/protein ratio was lower than that of the wild-type strain when grown in nutrient broth. However, when grown in minimal-glucose medium, the frequency of anucleate cells was reduced in all of them and the DNA/protein ratio was restored to normal. Especially, the oriC-deleted mutant in which the plasmid was integrated near oriC produced almost no anucleate cell. These results indicate that initiation frequency of chromosome replication from the integrated plasmid origin were reduced disproportionately to cell mass increase in rich medium, which in turn disrupted coordination between DNA replication cycle and cell division cycle. The locations of the plasmid origin relative to the natural oriC locus affected the production of anucleate cell remarkably, suggesting that partition mechanism of chromosome was also impaired by the translocation of its replication origin.

References Powered by Scopus

Identification of two new genes, mukE and mukF, involved in chromosome partitioning in Escherichia coli

116Citations
N/AReaders
Get full text

Chromosome partitioning in bacteria

49Citations
N/AReaders
Get full text

Chromosome partition in Echerichia coli

39Citations
N/AReaders
Get full text

Cited by Powered by Scopus

A Bacillus subtilis gene-encoding protein homologous to eukaryotic SMC motor protein is necessary for chromosome partition

171Citations
N/AReaders
Get full text

Dynamic Control of the DNA Replication Initiation Protein DnaA by Soj/ParA

167Citations
N/AReaders
Get full text

Dynamic localization of bacterial and plasmid chromosomes

136Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Moriya, S., Hassan, A. K. M., Kadoya, R., & Ogasawara, N. (1997). Mechanism of anucleate cell production in the oriC-deleted mutants of bacillus subtilis. DNA Research, 4(2), 115–126. https://doi.org/10.1093/dnares/4.2.115

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 5

38%

Researcher 5

38%

Professor / Associate Prof. 3

23%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 10

67%

Biochemistry, Genetics and Molecular Bi... 3

20%

Medicine and Dentistry 1

7%

Immunology and Microbiology 1

7%

Save time finding and organizing research with Mendeley

Sign up for free