Robust target labeling from small amounts of RNA for Illumina® Expression BeadChip® arrays

Abstract

The Illumina® Expression BeadChip® arrays are the preferred system for array-based gene expression studies because of their low input requirement, high sensitivity, low cost and high throughput. Two target labeling procedures—the TargetAmp™-Nano and TargetAmp™-Pico Target Labeling Kits for Illumina Expression BeadChip arrays—generate microgram amounts of biotin-antisense RNA (aRNA) from nanogram and picogram amounts of RNA, respectively, and yield high-quality expression analysis results.