TOTAL TEST OF Escherichia coli ON FRESH COW MILK AT DAIRY FARMER COOPERATIVE (KPSP) KARYO NGREMBOKO PURWOHARJO BANYUWANGI

Abstract

This study aims to measure the quality of fresh cow's milk deposited by farmers to the cooperative and then count the number of Escherichia coli bacteria which are then compared with SNI. Sampling is carried out when farmers deliver fresh cow's milk to the farmer cooperative. The samples taken were milk in milk can which was taken with sterile pipette and also the suction rubber. The next sample is stored in a sterile bottle and brought by coolbox frozen ice gel, then tested in the laboratory. Furthermore, planting on Lauryl Tryptose Broth (LTB), Escherichia coli Broth (ECB), Levine Eosin Methylen Blue Agar (L-EMBA) and biochemical test of Indole, Methyl Red, Voges Proskauer, Citrate (IMViC) using the Most Probable Number (MPN). The result showed that Escherichia coli bacteria exceeded the standard of SNI > 3 APM/ml 5 of 16 samples or 31,25%. The result of calculation based on MPN, sample 7 of 3 APM/ml, sample 9 is 3,6 APM/ml, sample 10 is 23 APM/ml, sample 13 is 7,4 APM/ml and sample 14 is 3,6 APM/ml.