In a separate chapter we describe a simple method for single nucleotide polymorphism (SNP) discovery using genomic reduction. Here we describe a scalable and cost-effective SNP genotyping method based on KBioscience’s competitive allele-specifi c PCR amplifi cation of target sequences and endpoint fl uorescence genotyping (KASPar™) using a FRET capable plate reader or Fluidigm’s dynamic array highthroughput platform.
CITATION STYLE
Smith, S. M., & Maughan, P. J. (2015). SNP genotyping using KASPar assays. Methods in Molecular Biology, 1245, 243–256. https://doi.org/10.1007/978-1-4939-1966-6_18
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