HPLC method for microanalysis and pharmacokinetics of marine sulfated polysaccharides, propylene glycol alginate sodium sulfate

Abstract

With the development of glycobiology and glycochemistry, bioactive polysaccharides are more and more widely used in the clinical area due to their anticoagulation, antitumor, immune regulation, antivirus, and other activities. As a marine sulfated polysaccharide, propylene glycol alginate sodium sulfate (PSS) has been commonly used as a heparinoid drug for the treatment of ischemic cerebrovascular and hyperlipidemia diseases in China for nearly 30 years. The reliable high-performance liquid chromatography (HPLC) with pre-column derivatization and post-column fluorescence derivatization methods is developed for the determination of the α-D-mannuronic acid L-guluronic acid (G) ratio of PSS and microanalysis of PSS in rat plasma, respectively. These methods provide the basis of the quality control, qualitative, and quantitative analysis. Especially, these methods can be used for microanalysis of other polysaccharide drugs in vivo and pharmacokinetic study.